loyalbahis güncel giriş Temel Açıklaması
loyalbahis güncel giriş Temel Açıklaması
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Our hypothesis was that, in the case of large biomolecules, the changes of molecular conformation caused by the pressure or high flow rates (thermal effects) will play a much more important role in changing retention and selectivity. Especially when considering their “bind and elute” type retention behavior. In our work, this huge conformational change was experimentally verified both by fluorescence emission measurements and by the analysis of native and reduced forms of various proteins which possess the same molecular weight but a different conformation.
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We saw that friction related effects were more important for small molecule separations whilst pressure effects were much more significant for protein separations. Insulin was an attractive example kakım we could clearly see the decrease of retention in constant inlet pressure mode and the increase of retention in variable inlet pressure mode when increasing the flow rate.
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In other work you have carried out fundamental studies in which the effect of longitudinal temperature gradient on retention, caused by frictional heating, was experimentally dissociated from the combined effect of pressure and frictional heating (3).
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